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In the current study we have used a whole genome sequencing (WGS) based approach to assess the level of sensitivity and specificity of class I and class II genotyping. We found that by using WGS it was possible to overcome the large allelic dropout rates commonly observed for the HLA-C locus [30]. Of note, the WGS based methods detect all of the HLA-C polymorphisms present in the samples, as they do not depend on TaqMan genotyping or using sequence-specific primers for amplification. Furthermore, the WGS based approach allows for simultaneous typing of the HLA-C locus alleles which is advantageous to South Korean population. Although the cost of the WGS based approach is higher than the TaqMan-based method, the method allows for unambiguous genotyping of the HLA-C locus. Moreover, while the specific-primers based approaches result in lower levels of concordance, they can be used for population level genotyping, and further algorithms can be used to identify the type of discrepancy [40]. By using a WGS based approach, we were able to determine the combination of HLA-C alleles present in over 150 samples, which could not have been achieved by using Ta
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